Journal: Cells

Article Title: Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model

doi: 10.3390/cells13060546

Figure Lengend Snippet: ( A ) Gene expression analysis for collagen 1 of chondrocyte spheroids and cartilage fragments in an inflammation model with TNFα from four independent donors and three technical replicates. Results are normalized to 18S as internal control, transformed by natural logarithm and visualized in box plots. ( B ) Immunohistochemical staining of chondrocyte spheroid constructs and cartilage fragments for collagen 1. Negative controls are shown in . ( C ) Semi-quantitative analysis of stain intensity of collagen 1 compared to background stain hematoxylin and eosin. * p < 0.05, ** p < 0.01, *** p < 0.001. P0, non-passaged chondrocytes; P3, chondrocyte passage 3; MCI, minced cartilage; TNFα, tumor necrosis factor α.

Article Snippet: The paraffin sections were stained with Weigert´s Haematoxylin (Sigma-Aldrich) for 10 min, followed by 0.02% Fast Green (Sigma-Alrich) in ultrapure (ddH 2 O) water for 6 min and 0.1% Safranin O for 10 min. Collagen type 1 and collagen type 2 immunohistochemistry was performed with the Vectastain avidin-biotin-complex based (Vector Laboratories, Newark, CA, USA) staining method, as previously described, with antibodies against collagen 1 (Arcis, Worrington, United Kingdom) or collagen 2 (DSHB, Iowa, IA, USA) and PBS as the negative control, followed by a biotinylated IgG antibody, DAB (3-3´-diaminobenzidine; Vector Laboratories).

Techniques: Expressing, Transformation Assay, Immunohistochemical staining, Staining, Construct